BD Matrigel™ Basement Membrane Matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins to include laminin (a major component), collagen IV, heparan sulfate proteoglycans, and entactin/nidogen.1,2 BD Matrigel Matrix also contains TGF-beta, epidermal growth factor, insulin-like growth factor, fibroblast growth factor, tissue plasminogen activator,3,4 and other growth factors which occur naturally in the EHS tumor. BD

BD™ Human Umbilical Vein Endothelial Cells (HUVEC-2) stained with Calcein AM and cultured on
BD Matrigel™ Matrix. Confocal image (4x) taken using the BD Pathway™ BioImager.

BD Matrigel™

Basement

Membrane

Matrix

BD Matrigel™ Basement Membrane Matrix is effective for the attachment and differentiation of both normal and transformed anchorage dependent epithelioid and other cell types. These include neurons,5,6 Sertoli cells,7 chick lens,8 and vascular endothelial cells,9 and hepatocytes.10 BD Matrigel Matrix will influence gene expression in adult rat hepatocytes11 as well as three dimensional culture in mouse12-15 and human16,17 mammary epithelial cells. It will support in vivo peripheral nerve regeneration,18-20 can be used for metabolism and toxicology studies,21,22 and is the basis for several types of tumor cell invasion assays.23,24 BD Matrigel Matrix provides the substrate necessary for the study of angiogenesis both in vitro25,26 and in vivo.27-29 BD Matrigel Matrix also supports in vivo propagation of human tumors in immunosupressed mice.30-32

Wide Selection of Basement

           Membrane Matrices:

BD Matrigel Matrix Growth Factor Reduced (GFR) is suited for applications where a more highly defined basement membrane preparation is desired. Available in standard GFR, High Concentration (HC), and Phenol Red-Free formats.

BD Matrigel Matrix High Concentration is suited for in vivo applications where a high protein concentration augments growth of tumors. The high protein concentration also allows the BD Matrigel Matrix Plug to maintain its integrity after subcutaneous injection into mice. Available in Standard, Growth Factor Reduced (GFR), and Phenol Red-Free formats.

BD Matrigel Matrix Phenol Red-Free is recommended for assays which require color detection (i.e. fluorescence).

BD Matrigel hESC-qualified Matrix has been qualified as mTeSR™1-compatible by StemCell Technologies, thus eliminating the need for time-consuming screening, in order to provide the reproducibility and consistency essential for your human embryonic stem (hES) cell research. The mTeSR1 formulation and BD Matrigel Matrix have been shown to be a successful combination for feeder-free maintenance of different WiCell™ hES cell lines for up to 20 passages (mTeSR1, StemCell Technologies Cat. No. 05850).

Typical Applications:

Cell Growth and Differentiation BD Matrigel Matrix is especially suited for the culture of polarized cells, such as epithelial cells. It promotes the differentiation of many cells types, including hepatocytes, neurons, mammary epithelial, endothelial, and smooth muscle cells.

In Vivo Angiogenesis Studies. BD Matrigel Matrix HC can be used to assess in vivo angiogenic activity of different compounds by subcutaneous injection into mice (BD Matrigel Plug Assay). The plugs are subsequently removed and analyzed for the formation of blood vessels.

Augmentation of Tumor Growth in Nude Mice.. BD Matrigel Matrix HC has been shown to promote successful transplantation of many human tumor cells, including prostatic, breast, small-cell lung, colon, adrenal carcinomas, melanomas, and lymphoblastic leukemia cells. Also, it has been found to increase tumor growth rates in vivo.

For literature citations and other BD™ Extracellular Matrix products, please visit bdbiosciences.com.

Quality Control:

1.Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing

2.Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process

3.Tested and found negative for bacteria, fungi, and mycoplasma

4.Protein concentrations are determined by Lowry method

5.Endotoxin units are measured by Limulus Amoebocyte Lysate assay

6.BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C

7.Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0 mm layer of BD Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor

Ordering Information